Prevalence of avian influenza viruses, Borrelia garinii, Mycobacterium avium, and Mycobacterium avium subsp. paratuberculosis in waterfowl and terrestrial birds in Slovakia, 2006

Paulina Gronesova (1) , Martina Ficova (1) , Adriana Mizakova (2) , Peter Kabat (1) , Alfred Trnka (3) , Tatiana Betakova (4)

1 Institute of Virology
2 Department of hygiene, epidemiology, veterinary provision and laboratory diagnostic
3 Trnava University
4 Institute of Virology

The prevalence of Borrelia (B.), Mycobacteria (M.) and avian influenza virus (AIV) infections, together with the distribution of different AIV subtypes, was studied in migratory waterfowl and terrestrial birds caught in three localities in Slovakia during 2006. Samples obtained from waterfowl captured in the Senianske Ponds area of Eastern Slovakia showed the highest diversity of AIV isolates. A total of 13 different subtypes were detected in 19 samples from this location (H1N2, H2N2, H3N2, H6N6, H7N6, H9N2, H9N5, H9N6, H10N5, H10N6, H12N6, H13N6, and H16N6). H3N5 virus was detected in 50% of passerines testing positive for AIV in the Parizske Wetlands, with H7N2, H9N2, H9N5, H12N1, and H13N2 infections also recorded at this locality. H9N5 virus predominated in passerines captured at Trnava Ponds, with isolates H1N6, H6N5, H7N2, H7N6, H10N3, H10N6 also detected at this location. There were five cases where different AIV infections were detected in oropharyngeal and cloacal samples originating from the same bird (H13N6 and H1N2; H10N5 and H12N6; H9N5 and H6N5; H10N6 and H7N6; H9N2 and H3N5 in the oropharynx and cloaca, respectively). Between 21 and 52% of captured birds tested positive for B. burgdorferi sensu lato, with the percentage infected depending on bird species and locality. Samples were characterized by PCR-RFLP analysis and identified as B. garinii species (either B/B' or R/R' pattern). Mycobacteria were detected in 42% and 26 % of waders captured at Senianske Ponds and marsh-dwelling passerines captured in the Parizske Wetlands, respectively. Interestingly, forest-dwelling passerine species caught in the Trnava Ponds region tested negative for Mycobacteria.

Mots clés: Life Sciences

Paulina Gronesova, Martina Ficova, Adriana Mizakova, Peter Kabat, Alfred Trnka, et al.. Prevalence of avian influenza viruses, Borrelia garinii, Mycobacterium avium, and Mycobacterium avium subsp. paratuberculosis in waterfowl and terrestrial birds in Slovakia, 2006. Avian Pathology, 2008, 37 (05), pp.537-543. ⟨10.1080/03079450802356953⟩. ⟨hal-00540129⟩