Genotyping of Histomonas meleagridis isolates based on Internal Transcribed Spacer-1 (ITS-1) sequences

Harold M.J.F. van Der Heijden (1) , Wil J.M. Landman (2) , Sophie Greve (3) , Ron Peek (3)

1 R&D
2 Poultry Health
3 Section of Parasitology, Department of Medical Microbiology

C-profiling is a novel genotyping method for protozoan pathogens, based on PCR and sequencing of AT-rich ITS-1 sequences. It was applied to various Histomonas meleagridis isolates originating from outbreaks of histomoniasis in six Dutch turkey and chicken flocks. Three different H. meleagridis genotypes were identified. Type I and II were associated with clinical disease. In two flocks both having recovered from an outbreak of histomoniasis, a type III strain was found that was also morphologically slightly different from the type I and II isolates. C-profiling is a promising technique to differentiate between H. meleagridis subtypes making it useful for epidemiological studies.

Mots clés: Life Sciences

Harold M.J.F. van Der Heijden, Wil J.M. Landman, Sophie Greve, Ron Peek. Genotyping of Histomonas meleagridis isolates based on Internal Transcribed Spacer-1 (ITS-1) sequences. Avian Pathology, 2006, 35 (04), pp.330-334. ⟨10.1080/03079450600815499⟩. ⟨hal-00540049⟩