Changes in the gene expression after short-term ex vivo therapy with prednisolone have prognostic impact in childhood acute lymphoblastic leukemia

Hanna Janiszewska (1) , Jan Styczynski (2) , Beata Kolodziej (2) , Mariusz Wysocki (2) , Olga Haus (1)

1 Department of Clinical Genetics, Collegium Medicum
2 Department of Pediatric Hematology and Oncology, Collegium Medicum

Multidrug resistance 1 () gene expression determined by real-time polymerase chain reaction and results of rhodamine assay were analyzed at diagnosis and after 3 days of ex vivo therapy with prednisolone in 36 pediatric patients with acute lymphoblastic leukemia (ALL). Only 62% patients with de novo ALL had significant decrease of expression. These patients had over twofold lower rhodamine retention in the presence of cyclosporine A on day 3 than others and had better probability of disease-free survival. In this study, we have shown that changes in the expression of gene after short-term incubation of lymphoblasts with prednisolone may have prognostic value in pediatric de novo ALL patients.

Mots clés: Childhood ALL; Ex vivo therapy with prednisolone; expression; Real-time PCR

Hanna Janiszewska, Jan Styczynski, Beata Kolodziej, Mariusz Wysocki, Olga Haus. Changes in the gene expression after short-term ex vivo therapy with prednisolone have prognostic impact in childhood acute lymphoblastic leukemia. Annals of Hematology, 2009, 88 (12), pp.1193-1198. ⟨10.1007/s00277-009-0739-1⟩. ⟨hal-00535051⟩