Identification and biophysical assessment of the molecular recognition mechanisms between the human hemopoietic cell kinase Src homology domain 3 and ALG-2-interacting protein X - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biochemical Journal Année : 2010

Identification and biophysical assessment of the molecular recognition mechanisms between the human hemopoietic cell kinase Src homology domain 3 and ALG-2-interacting protein X

Xiaoli Shi
  • Fonction : Auteur
Sandrine Opi
  • Fonction : Auteur
Adrien Lugari
  • Fonction : Auteur
Audrey Restouin
  • Fonction : Auteur
Thibault Coursindel
  • Fonction : Auteur
Isabelle Parrot
Javier Perez
  • Fonction : Auteur
Eric Madore
  • Fonction : Auteur
Pascale Zimmermann
  • Fonction : Auteur
Jacques Corbeil
  • Fonction : Auteur
Mingdong Huang
  • Fonction : Auteur
Stefan T Arold
  • Fonction : Auteur
Yves Collette

Résumé

Src family kinases (SFKs) are central regulators of many signaling pathways. Their functions are tightly regulated through SH (Src homology) domain-mediated protein–protein interactions. A yeast two-hybrid screen using SH3 domains as bait identified Alix (ALG-2 [apoptosis-linked gene 2]-interacting protein X) as a novel Hck (hemopoietic cell kinase) SH3 domain interactor. The Alix–Hck-SH3 interaction was confirmed in vitro by a GST (glutathione S-transferase) pull-down assay and in intact cells by a mammalian two-hybrid assay. Furthermore, the interaction was demonstrated to be biologically relevant in cellulo. Through biophysical experiments, we then identified the PRR (proline-rich region) motif of Alix that binds Hck-SH3 with a dissociation constant of 34.5 µM. Heteronuclear NMR spectroscopy experiments was used to map Hck-SH3 residues that interact with the ALIXV+PRR construct or with the minimum identified interacting motif. Finally, small-angle X-ray scattering (SAXS) analysis showed that the N-terminal PRR of Alix is unfolded, at least before Hck-SH3 recognition. Our results indicate that residues outside the canonical PxxP motif of Alix enhance its affinity and selectivity toward Hck-SH3. The structural framework of the Hck–Alix interaction demonstrated here will help in clarifying how Hck and Alix assist during virus budding and cell surface receptor regulation.

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hal-00517249 , version 1 (14-09-2010)

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Xiaoli Shi, Sandrine Opi, Adrien Lugari, Audrey Restouin, Thibault Coursindel, et al.. Identification and biophysical assessment of the molecular recognition mechanisms between the human hemopoietic cell kinase Src homology domain 3 and ALG-2-interacting protein X. Biochemical Journal, 2010, 431 (1), pp.93-102. ⟨10.1042/BJ20100314⟩. ⟨hal-00517249⟩

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