A PHYSICAL STUDY OF DYNAMICS IN FULLY HYDRATED PHOSPHOLIPID BILAYERS

Giovanna d'Angelo (1) , Ulderico Wanderlingh (2) , Valeria Conti Nibali (2) , Cristina Crupi (2) , Carmelo Corsaro (3) , G. Di Marco (4)

1 physics
2 physics
3 Physics
4 IPCF - CNR Istituto per i Processi Chimico-Fisici

We have performed a study concerning the thermotropic phase behavior of phospholipid membrane bilayer of dimyristoylphosphatidylcholine (DMPC) in excess of water condition. We have also investigated the effect induced by the antimicrobial peptide Gramicidin D (GrD) inserted into the membrane bilayer. This study was carried out by using several techniques in order to collect information on different properties and on different spatial and temporal regimes. We have in fact used Differential Scanning Calorimetry (DSC), Fixed Energy Window (FEW) neutron scattering, Pulsed Field Gradient Nuclear Magnetic Resonance (NMR) and Molecular Dynamics (MD) simulations. We have obtained information on the main phase transition (gel – liquid crystalline) temperature of bilayer system and on long range diffusion coefficient for both phospholipids and hydration water. Moreover, we have evidenced the effect of the transition on the microscopic molecular mobility perpendicular and parallel to the membrane plane. The influence of gramicidin on these properties are also commented.

Mots clés: Physical Sciences

Giovanna d'Angelo, Ulderico Wanderlingh, Valeria Conti Nibali, Cristina Crupi, Carmelo Corsaro, et al.. A PHYSICAL STUDY OF DYNAMICS IN FULLY HYDRATED PHOSPHOLIPID BILAYERS. Philosophical Magazine, 2008, 88 (33-35), pp.4033-4046. ⟨10.1080/14786430802609651⟩. ⟨hal-00514003⟩