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Article Dans Une Revue Clinical Science Année : 2009

Eicosapentaenoic acid stimulates AMP-activated protein kinase and increases visfatin secretion in cultured murine adipocytes

Silvia Lorente-Cebrián
  • Fonction : Auteur
Matilde Bustos
  • Fonction : Auteur
Amelia Marti
  • Fonction : Auteur
J Alfredo Martínez
  • Fonction : Auteur

Résumé

Visfatin is an adipokine highly expressed in visceral adipose tissue (AT) of humans and rodents, the production of which seems to be dysregulated in excessive fat accumulation and conditions of insulin resistance. Eicosapentaenoic acid (EPA), an n-3 polyunsaturated fatty acid (PUFA), has been demonstrated to exert beneficial effects within obesity and insulin resistance conditions, which have been further linked to its reported ability to modulate adipokine production by adipocytes. TNF-α is a pro-inflammatory cytokine whose production is increased in obesity and involved in the development of insulin resistance. Control of adipokine production by some insulin-sensitizing compounds has been associated with the stimulation of AMP-activated protein kinase (AMPK). The aim of this study was to examine in vitro the effects of EPA on visfatin production and the potential involvement of AMPK both in the absence or presence of TNF-α Treatment with the pro-inflammatory cytokine TNF-α (1 ng/mL) did not modify visfatin gene expression and protein secretion in primary cultured rat adipocytes. However, treatment of these primary adipocytes with EPA (200 µM) for 24 h significantly increased visfatin secretion (p<0.001) and mRNA gene expression (p<0.05). Moreover, the stimulatory effect of EPA on visfatin secretion was prevented by treatment with AMPK inhibitor Compound C but not with phosphatidylinositol 3-kinase (PI3K) inhibitor LY 294002. Similar results were observed in 3T3-L1 adipocytes. Moreover, EPA strongly stimulated AMPK phosphorylation alone or in combination with TNF-α in 3T3-L1 adipocytes and pre-adipocytes. These results suggest that the stimulatory action of EPA on visfatin production involves AMPK activation in adipocytes.

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hal-00509663 , version 1 (14-08-2010)

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Silvia Lorente-Cebrián, Matilde Bustos, Amelia Marti, J Alfredo Martínez, Maria J. Moreno-Aliaga. Eicosapentaenoic acid stimulates AMP-activated protein kinase and increases visfatin secretion in cultured murine adipocytes. Clinical Science, 2009, 117 (6), pp.243-249. ⟨10.1042/CS20090020⟩. ⟨hal-00509663⟩

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