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| HAL : hal-00506388, version 1 |
| PubMed : 19934032 |
| PubMed Central : 2795548 |
| DOI : 10.1073/pnas.0909893106 |
| Fiche détaillée |  Récupérer au format |
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| Proceedings of the National Academy of Sciences of the United States of America 106, 50 (2009) 21155-60 |
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| Acidianus filamentous virus 1 coat proteins display a helical fold spanning the filamentous archaeal viruses lineage. |
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| Adeline GouletStéphanie Blangy |
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| (15/12/2009) |
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| Acidianus filamentous virus 1 (AFV1), a member of the Lipothrixviridae family, infects the hyperthermophilic, acidophilic crenarchaeaon Acidianus hospitalis. The virion, covered with a lipidic outer shell, is 9,100-A long and contains a 20.8-kb linear dsDNA genome. We have identified the two major coat proteins of the virion (MCPs; 132 and 140 amino acids). They bind DNA and form filaments when incubated with linear dsDNA. A C-terminal domain is identified in their crystal structure with a four-helix-bundle fold. In the topological model of the virion filament core, the genomic dsDNA superhelix wraps around the AFV1-132 basic protein, and the AFV1-140 basic N terminus binds genomic DNA, while its lipophilic C-terminal domain is imbedded in the lipidic outer shell. The four-helix bundle fold of the MCPs from AFV1 is identical to that of the coat protein (CP) of Sulfolobus islandicus rod-shaped virus (SIRV), a member of the Rudiviridae family. Despite low sequence identity between these proteins, their high degree of structural similarity suggests that they could have derived from a common ancestor and could thus define an yet undescribed viral lineage. |
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| 1 : | Biologie Moléculaire du Gène chez les Extrêmophiles (BMGE) |
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Institut Pasteur de Paris |
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| 2 : | Institut de génétique et microbiologie (IGM) |
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CNRS : UMR8621 – Université Paris XI - Paris Sud |
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| 3 : | Architecture et fonction des macromolécules biologiques (AFMB) |
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CNRS : UMR6098 – Université de la Méditerranée - Aix-Marseille II – Université de Provence - Aix-Marseille I |
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| Domaine | : | Sciences du Vivant/Biochimie, Biologie Moléculaire Sciences du Vivant/Microbiologie et Parasitologie |
| hal-00506388, version 1 | |
| http://hal.archives-ouvertes.fr/hal-00506388 | |
| oai:hal.archives-ouvertes.fr:hal-00506388 | |
| Contributeur : Stephanie Beaunay | |
| Soumis le : Mardi 27 Juillet 2010, 15:40:44 | |
| Dernière modification le : Mardi 27 Juillet 2010, 15:40:44 | |
