Interleukin-1 system in the human fallopian tube - no spatial but a temporal regulation of mRNA and protein expression

A.P. Hess , D.M. Baston-Buest , A. Schanz , J. Hirchenhain , P. Bielfeld , J.S. Kruessel

The human fallopian tube provides the environment for the first 5 days of embryonic development . The IL-1 system is involved in human embryo implantation. This study aimed to investigate IL-1β, IL-1ra and IL-1R tI expression within the length of the human fallopian tube on mRNA- and protein-level in samples from proliferative versus secretory phase, postmenopause samples (PMP) and samples from intra- (IUP) and extrauterine (EUP) pregnancies to examine possible spatial and hormonal induced changes (fimbrial, ampullary and isthmic tube segments). On mRNA-level, IL-1β was expressed in all samples except in PMP. IL-1R tI could be detected in all samples whereas IL-1ra was only expressed in secretory phase and the IUP sample. Immunohistochemically we could detect IL-1β and IL-1R t1 protein in all proliferative and secretory phase samples with maximum intensity in secretory phase samples whereas IL-1ra was expressed in secretory phase samples only. Overall no spatial but temporal differences possibly due to hormonal changes could be observed suggesting a precise regulation of the IL-1 system, especially for IL-1ra and moreover a stable molecular architecture within the full length of the fallopian tube.

Mots clés: Interleukin-1 (IL-1) system; Human fallopian tube; Interleukin-1 receptor type I (IL-1R tI); Interleukin-1 receptor antagonist (IL-1ra); Implantation; Spatial and temporal expression pattern

A.P. Hess, D.M. Baston-Buest, A. Schanz, J. Hirchenhain, P. Bielfeld, et al.. Interleukin-1 system in the human fallopian tube - no spatial but a temporal regulation of mRNA and protein expression. Molecular and Cellular Endocrinology, 2009, 303 (1-2), pp.7. ⟨10.1016/j.mce.2009.01.017⟩. ⟨hal-00499112⟩