Origin of the nitrogen assimilated by soil fauna living in decomposing beech litter

Laurent Caner (1) , Bernhard Zeller (1) , Etienne E. Dambrine (1, 1) , Jean-François Ponge (2) , Matthieu Chauvat (3) , Curmi Llanque (1)

1 BEF - Unité de recherche Biogéochimie des Ecosystèmes Forestiers
2 ECOTROP - Fonctionnement, évolution et mécanismes régulateurs des écosystèmes forestiers
3 Department of Animal Ecology and Systematics

We investigated the nitrogen source for main taxa of soil fauna in two beech forests of contrasted humus type using N-15-labelled beech litter and N-15 analysis of soil fauna. N-15-labelled beech litter was deposited on the topsoil in December 2000 in four stands of different ages at Leinefelde (Germany) with mull humus and in one mature stand at Sorphi (Denmark) with moder humus. The fate of the tracer isotope was measured in litter and soil, as well as in the soil fauna, and for each taxa, we calculated the proportion of N in the animal derived from the labelled substrate. Of the original N contained in the litter, 20-41% was lost after 9 months at Leinefelde, and only 10% at Sorphi. This loss was counterbalanced by the incorporation of 24-31% external N at Leinefelde, and 31% at Sorphi, partly originating from fungal colonisation of the added litter. The proportion of N assimilated from the labelled litter by the different soil animals varied in relation to their mobility and feeding preferences. Large and mobile soil animals, especially predators, derived on average less N-15 because they were also able to feed outside the labelled litter boxes. Detritivores assimilated at most 15% of their nitrogen content at Leinefelde and 11% at Sorphi from the decomposing labelled litter. The most labelled taxa at Leinefelde were small fungivorous and coprophagous species, mainly isotomid Collembola such as Isotomiella and Folsomia. At Sorphi, best labelled taxa were saprophagous species such as Enchytraeidae, Glomeridae and Phthiracaroidea. These low rates of N-15 assimilation indicate that fresh litter is not directly the main N source for soil animals. The results obtained suggest that soil fauna fed preferentially upon microorganisms colonising the litter at Leinefelde (mull) and from litter itself at Sorphi (moder).

Mots clés: N-15 tracing; Litter; Soil fauna; Detritivores; Predators

Domaines: Science des sols

Laurent Caner, Bernhard Zeller, Etienne E. Dambrine, Jean-François Ponge, Matthieu Chauvat, et al.. Origin of the nitrogen assimilated by soil fauna living in decomposing beech litter. Soil Biology and Biochemistry, 2004, 36 (11), pp.1861-1872. ⟨10.1016/j.soilbio.2004.05.007⟩. ⟨hal-00497766v2⟩