Intra-species growth-inhibition by is a possible virulence trait in necrotic enteritis in broilers

Leen Timbermont , Anouk Lanckriet , Frank Pasmans , Freddy Haesebrouck , Richard Ducatelle , Filip van Immerseel

Necrotic enteritis in broiler chickens is associated with type A, carrying the NetB toxin. C. type A is also a member of the normal intestinal microbiota of broilers. Clinically healthy chickens carry several different clones in their intestine. In flocks suffering from necrotic enteritis, however, mostly only one single clone is isolated from the gut of all the diseased animals. Selective proliferation of these clinical outbreak strains in the gut and spread within the flock seems likely, but an explanation has not yet been given. The hypothesis that necrotic enteritis associated strains might suppress the growth of normal microbiota strains, was therefore tested. Twenty-six strains isolated from healthy broilers and 24 clinical outbreak isolates were evaluated for their ability to induce intra-species growth-inhibition in an setup. A significantly higher proportion of the clinical outbreak strains inhibited the growth of other strains compared to strains isolated from the gut of healthy chickens. It is proposed that, in addition to toxin production, intra-species inhibition may be a virulence trait that contributes to the ability of certain strains to cause necrotic enteritis in broilers.

Mots clés: Broiler; Necrotic enteritis; Growth-inhibition

Leen Timbermont, Anouk Lanckriet, Frank Pasmans, Freddy Haesebrouck, Richard Ducatelle, et al.. Intra-species growth-inhibition by is a possible virulence trait in necrotic enteritis in broilers. Veterinary Microbiology, 2009, 137 (3-4), pp.388. ⟨10.1016/j.vetmic.2009.01.017⟩. ⟨hal-00485533⟩