Molecular characterisation of strains isolated from small and large ruminants reveals a host rather than tissue specificity

P.D.D. Alves , J.A. Mcculloch , Sergine Even , C. Le Maréchal , A. Thierry , N. Grosset , V. Azevedo , C.A. Rosa , E. Vautor , Yves Le Loir

is an important pathogen in domestic ruminants. The main objective of this study was to determine the similarity of epidemiologically unrelated . isolates from bovine, ovine, and caprine hosts regardless the locus of isolation (nares and udder). By pulsed-field gel electrophoresis, 7 major pulsotypes were identified among 153 isolates recovered from 12 different regions of France as well as from Brazil, the USA and Belgium. Typing of the accessory gene regulator () and capsular () serotype was carried out on all the isolates and revealed the predominance of I and III and of regardless the ruminant host species. Screening for MRSA was carried out by disk diffusion and revealed a prevalence of only 3.2% of MRSA among the strains tested. These results suggest the existence of a host rather than tissue specificity among isolates colonising the ruminant species and suggest a limited transmission of those isolates between large (bovine) and small (ovine-caprine) ruminants. The class and types correlated with pulsotype clusters rather than with a specific host species. Antimicrobial resistance appears not to have contributed to the predominance of any given genotypes, and MRSA prevalence appears very low in ruminant isolates.

Mots clés: Staphylococcus aureus; Ruminant; host specificity; mastitis; PFGE

P.D.D. Alves, J.A. Mcculloch, Sergine Even, C. Le Maréchal, A. Thierry, et al.. Molecular characterisation of strains isolated from small and large ruminants reveals a host rather than tissue specificity. Veterinary Microbiology, 2009, 137 (1-2), pp.190. ⟨10.1016/j.vetmic.2008.12.014⟩. ⟨hal-00485522⟩