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Article Dans Une Revue Breast Cancer Research and Treatment Année : 2009

Parvovirus H-1 induces cytopathic effects in breast carcinoma-derived cultures

Résumé

Parvovirus H-1 (H-1 PV) preferentially replicates in malignant cells resulting in their death by cytolysis. It has often been considered a potential candidate for use in novel anticancer therapy. To evaluate its potential in a model of natural tumors, we assayed in vitro the effect exerted by H-1 PV on short-term cultures derived from breast tumor samples freshly excised from patients. Our results show that H-1 PV effectively kills tumor-derived cells, whereas normal tissue-derived cells showed no H-1 PV-induced cytopathic effects (CPE). We also determined that the H-1 PV sensitivity (up to 67% sensitive cultures) is related with the quantities of virus assayed. We further examined the expression and phosphorylation state of the parvoviral nonstructural protein 1 (NS1), known to be associated with parvoviruses-induced CPE. Both appear to be impaired in normal tissue-derived cells and resistant cultures. Finally, we show that H-1 PV sensitivity in cultures correlates significantly with higher tumor grades (Nottingham combined histologic grade 2 or 3). This report confirms that H-1 PV can efficiently induce CPE in primary breast tumor cells in vitro. It identifies tumor characteristics representing potential criteria for recruiting patients for clinical evaluation of H-1 PV antitumor effects.
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Dates et versions

hal-00485061 , version 1 (20-05-2010)

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Ghaffar Muharram, Emilie Le Rhun, Ingrid Loison, Pierre Wizla, Audrey Richard, et al.. Parvovirus H-1 induces cytopathic effects in breast carcinoma-derived cultures. Breast Cancer Research and Treatment, 2009, 121 (1), pp.23-33. ⟨10.1007/s10549-009-0451-9⟩. ⟨hal-00485061⟩
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