Palytoxins specific and dynamic detection by in vitro microplate assay with human neuroblastoma cells

Begoña Espiña , Eva Cagide , M. Carmen Louzao (1) , Maria Martinez-Fernandez , Mercedes R Vieytes , Panagiota Katikou , Adriano Villar , David Jaen , Luz Maman , Luis M Botana

1 Departamento de Farmacologia

Palytoxin is one of the most complex and biggest molecules known showing extreme acute toxicity. During the last years, the dinoflagellate Ostreopsis spp, the producer organism of palytoxin, has shown a world-wide distribution thus making palytoxin an emerging toxin. Rat derived hepatocytes Clone 9 and BE (2)-M17 human neuroblastoma were used to test palytoxin or palytoxin -like compounds by measuring cell metabolic rate with Alamar Blue. The dose-dependent decrease in viability was specifically inhibited by ouabain in the case of BE (2)-M17 neuroblastoma cells. This is a functional, dynamic and simple test for palytoxins with high sensitivity as low as 0.2 ng/ mL. This method was useful for toxin detection in Ostreopsis extracts and naturally contaminated mussel samples. A comparative study testing toxic mussel extracts by LC-MS/MS, MBA, hemolysis neutralization assay and cytotoxicity test indicated that our method is suitable for the routine determination and monitoring of palytoxins and palytoxin-like compounds.

Mots clés: Life Sciences

Begoña Espiña, Eva Cagide, M. Carmen Louzao, Maria Martinez-Fernandez, Mercedes R Vieytes, et al.. Palytoxins specific and dynamic detection by in vitro microplate assay with human neuroblastoma cells. Bioscience Reports, 2008, 29 (1), pp.13-23. ⟨10.1042/BSR20080080⟩. ⟨hal-00479303⟩