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Article Dans Une Revue Biochemical Journal Année : 2010

Bioinformatic and experimental survey of 14-3-3 binding sites

Catherine Johnson
Sandra Crowther
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Margaret Stafford
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David G Campbell
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Rachel Toth
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Carol Mackintosh
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Résumé

>200 phosphorylated 14-3-3-binding sites in the literature were analyzed to define 14-3-3 specificities, identify relevant protein kinases, and give insights into how cellular 14-3-3/phosphoprotein networks work. Mode I RXX(pS/pT)XP motifs dominate though the +2 proline occurs in less than half, and LX(R/K)SX(pS/pT)XP is prominent in plant 14-3-3-binding sites. Proline at +1 is rarely reported, and such motifs did not stand up to experimental reanalysis of human Ndel1. Instead, we discovered that 14-3-3 interacts with two residues that are phosphorylated by basophilic kinases and located in the disrupted-in-schizophrenia 1 (DISC1)-interacting region of Ndel1 that is implicated in cognitive disorders. These data conform with the general findings that there are different subtypes of 14-3-3-binding sites that overlap with the specificities of different basophilic AGC and CAMK protein kinases, and a 14-3-3 dimer often engages with two tandem phosphorylated sites, which is a configuration with special signalling, mechanical and evolutionary properties. Thus, 14-3-3 dimers can be digital logic gates that integrate more than one input to generate an action, and coincidence detectors when the two binding sites are phosphorylated by different protein kinases. Paired sites are generally located within disordered regions and/or straddle either side of functional domains, indicating how 14-3-3 dimers modulate the conformations and/or interactions of their targets. Finally, 14-3-3s bind to members of several multi-protein families. Two 14-3-3-binding sites are conserved across the class IIa histone deacetylases, whereas other protein families display differential regulation by 14-3-3s. We speculate that 14-3-3 dimers may have contributed to the evolution of such families, tailoring regulatory inputs to different physiological demands.

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Dates et versions

hal-00479284 , version 1 (30-04-2010)

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Catherine Johnson, Sandra Crowther, Margaret Stafford, David G Campbell, Rachel Toth, et al.. Bioinformatic and experimental survey of 14-3-3 binding sites. Biochemical Journal, 2010, 427 (1), pp.69-78. ⟨10.1042/BJ20091834⟩. ⟨hal-00479284⟩

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