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Article Dans Une Revue Biochemical Journal Année : 2008

Plant plasma membrane water channels conduct the signaling molecule H2O2

Marek Dynowski
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Gabriel Schaaf
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Dominique Loque
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Oscar Moran
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Uwe Ludewig
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Résumé

Hydrogen peroxide (H2O2) is a relatively long-lived reactive oxygen species that signals between cells and organisms. H2O2 signaling in plants is essential for response to stress, defense against pathogens, and the regulation of programmed cell death. Although H2O2 diffusion across membranes is often considered as a passive property of lipid bilayers, native membranes represent significant barriers for hydrogen peroxide. We addressed the question of whether channels might facilitate H2O2 transport across plasma membranes. The expression of several plant plasma membrane aquaporins in yeast, including PIP2;1 from Arabidopsis, enhanced the toxicity of H2O2 and increased the fluorescence of dye-loaded yeast when exposed to H2O2. The sensitivity of aquaporin-expressing yeast to hydrogen peroxide was altered by mutations that alter gating and the selectivity of the aquaporins. The transport of water, H2O2 and urea was compared, using molecular dynamics simulations based on the crystal structure of SoPIP2;1 from spinach. The calculations identify differences in the conduction between the substrates and reveal channel residues critically involved in H2O2 conduction. The results of the calculations on tetramers and monomers are in agreement with the biochemical data. Taken together, the data strongly postulate that plasma membrane aquaporin pores determine the efficiency of H2O2-signaling between cells. Aquaporins are present in most species and their capacity to facilitate the diffusion of H2O2 may be of physiological significance in many organisms and particularly in communication between different species.

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Dates et versions

hal-00478969 , version 1 (30-04-2010)

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Marek Dynowski, Gabriel Schaaf, Dominique Loque, Oscar Moran, Uwe Ludewig. Plant plasma membrane water channels conduct the signaling molecule H2O2. Biochemical Journal, 2008, 414 (1), pp.53-61. ⟨10.1042/BJ20080287⟩. ⟨hal-00478969⟩

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