Chaperone ligand-discrimination by the TPR-Domain Protein Tah1

Stefan H Millson , Cara K. Vaughan , Chao Zhai , Maruf M. U. Ali , Barry Panaretou , Peter W. Piper , Laurence H. Pearl , Chrisostomos Prodromou (1)

1 Section of Structural Biology

Tah1 has been identified as a tetratricopeptide (TPR)-domain protein. TPR-domain proteins are involved in protein-protein interactions and a number have been characterized that interact either with Hsp70 or Hsp90, but a few can bind both chaperones. Independent studies suggest that Tah1 interacts with Hsp90, but whether it can also interact with Hsp70/Ssa1 has not been investigated. Amino acid sequence alignments suggest that Tah1 is most similar to the TPR2b-domain of Hop which when mutated reduces binding to both Hsp90 and Hsp70. Our alignments suggest that there are three TPR-domain motifs in Tah1 which is consistent with the architecture of the TPR2b-domain. We find that Tah1 is specific for Hsp90, able to bind tightly the yeast Hsp90, and the human Hsp90α and Hsp90β proteins, but not the yeast Hsp70, Ssa1 isoform. Tah1 acheives ligand discrimination by favourably binding the methionine residue in the conserved MEEVD-motif (Hsp90) and positively discriminating against the first valine residue in the VEEVD-motif (Ssa1). We also show that Tah1 can affect the ATPase activity of Hsp90, in common with some other TPR-domain proteins.

Mots clés: Life Sciences

Stefan H Millson, Cara K. Vaughan, Chao Zhai, Maruf M. U. Ali, Barry Panaretou, et al.. Chaperone ligand-discrimination by the TPR-Domain Protein Tah1. Biochemical Journal, 2008, 413 (2), pp.261-268. ⟨10.1042/BJ20080105⟩. ⟨hal-00478953⟩