Identification of protein-ribulosamine-5-phosphatase as human low-molecular-weight protein tyrosine phosphatase-A - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biochemical Journal Année : 2007

Identification of protein-ribulosamine-5-phosphatase as human low-molecular-weight protein tyrosine phosphatase-A

Juliette Fortpied
  • Fonction : Auteur
Rita Gemayel
  • Fonction : Auteur
Didier Vertommen

Résumé

Ribulosamines, which are substrates for the deglycating enzyme fructosamine-3-kinase-related protein, are presumably formed intracellularly through glycation of proteins with ribose 5-phosphate followed by dephosphorylation of resulting ribulosamine 5-phosphates by a putative ribulosamine-5-phosphatase. Ribose 5-phosphate is known to be a potent glycating agent and we show in the present work that it reacts ≈ 10- and 80-fold more rapidly with protein than ribose and glucose, respectively. We also show that tissue and most particularly erythrocyte extracts contain a protein-ribulosamine-5-phosphatase. We have purified this enzyme from human erythrocytes to near-homogeneity and shown it to correspond to 'low-molecular-weight protein-tyrosine-phosphatase-A'. Human recombinant low-molecular-weight protein-tyrosine-phosphatase-A displayed a ribulosamine-5-phosphatase activity that was higher than its tyrosine-phosphatase activity, indicating that this phosphatase may participate in protein deglycation, a new form of protein repair.

Mots clés

Fichier principal
Vignette du fichier
PEER_stage2_10.1042%2FBJ20061485.pdf (670.56 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-00478675 , version 1 (30-04-2010)

Identifiants

Citer

Juliette Fortpied, Rita Gemayel, Didier Vertommen, Emile van Schaftingen. Identification of protein-ribulosamine-5-phosphatase as human low-molecular-weight protein tyrosine phosphatase-A. Biochemical Journal, 2007, 406 (1), pp.139-145. ⟨10.1042/BJ20061485⟩. ⟨hal-00478675⟩

Collections

PEER
35 Consultations
61 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More