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Article Dans Une Revue Biochemical Journal Année : 2006

Prolactin stimulates prostate cell proliferation by increasing endoplasmic reticulum content due to SERCA 2b over-expression

Alexandre Crépin
  • Fonction : Auteur
Gabriel Bidaux
Fabien Vanden-Abeele
  • Fonction : Auteur
Etienne Dewailly
  • Fonction : Auteur
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Vincent Goffin
Natalia Prevarskaya

Résumé

Prolactin (PRL) has been shown to be involved in the differentiation and proliferation of numerous tissues, including the prostate gland. Moreover, variations in calcium concentration within the endoplasmic reticulum ([Ca 2+}] ER}) may play a role in cell growth. However, few studies have focused on the regulation of calcium homeostasis by prolactin. This study evaluates the regulation of calcium pools as well as the possible role of [Ca 2+}] ER} variations as a signal for growth modulation by PRL. We show that PRL stimulates the proliferation of normal SV40 immortalized epithelial prostatic (PNT1A) cells with a maximum effect at a dose of 100 ng/ml. We also show that PRL (100 ng/ml) increases the [Ca 2+}] ER} when measured either by indirect quantification with FURA-2AM after application of 1 µM thapsigargin or by direct quantification with MagFURA-2AM within the endoplasmic reticulum. Western blot analysis shows that the sarco-endoplasmic calcium ATPase 2b (SERCA 2b) is over-expressed in PNT1A cells treated with 100 ng/ml PRL for 24 hours. A siRNA SERCA2a/b, used to downregulate endogenous SERCA 2b expression, reduces both PNT1A cell proliferation and [Ca 2+}] ER}. We thus identify [Ca 2+}] ER} and SERCA 2b as protagonists in PRL-induced proliferation.

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Dates et versions

hal-00478615 , version 1 (30-04-2010)

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Alexandre Crépin, Gabriel Bidaux, Fabien Vanden-Abeele, Etienne Dewailly, Vincent Goffin, et al.. Prolactin stimulates prostate cell proliferation by increasing endoplasmic reticulum content due to SERCA 2b over-expression. Biochemical Journal, 2006, 401 (1), pp.49-55. ⟨10.1042/BJ20060870⟩. ⟨hal-00478615⟩

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