MEGF9: a novel transmembrane protein with a strong and developmentally regulated expression in the nervous system
Résumé
EGFL5, a novel transmembrane protein with multiple Epidermal Growth Factor (EGF)-like repeats is predominantly expressed in the developing and adult central and peripheral nervous system. The domain structure of EGFL5 consists of an N-terminal region with several potential O-glycosylation sites followed by five EGF-like domains, which are highly homologous to the short arms of laminins. Following one single pass transmembrane domain, a highly conserved short intracellular domain with potential phosphorylation sites is present. The protein was recombinantly expressed and characterized as a tissue component. To further study the expression pattern, immunohistochemistry was performed and staining was detected in Purkinje cells of the cerebellum and in glial cells of the peripheral nervous system. Additional expression was observed in the epidermal layer of skin, papillae of the tongue and the epithelium of the gastrointestinal tract. By immunoelectron microscopy, EGFL5 was detected in glial cells of the sciatic nerve facing the basement membrane. EGFL5 represents a novel putative receptor, expressed in neuronal and non-neuronal tissues, that is regulated during development and could function as a guidance or signaling molecule.
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