New test for the diagnosis of bacterial endophthalmitis

Pablo Goldschmidt (1) , Sandrine Degorge (1) , Djida Benallaoua (1) , Elena Basli (1) , Laurence Batellier (1) , Sandrine Boutboul (1) , Cécile Allouch (1) , Vincent M. Borderie (1) , Laurent Laroche (1) , Christine Chaumeil (1)

1 CHNO - Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts

Diagnosis of bacterial endophthalmitis (BE) often fails due to: 1-reduced volumes of vitreous fluid (VF) and aqueous humor (AH); 2-lack of sensitivity of culture; 3-antibiotic treatments; 4-PCR cross-contamination and 5-limitations for real-time PCR melting curve interpretation. We developed an fast real-time-PCR to improve laboratory diagnosis performances.

Mots clés: Aqueous humour; Vitreous; Retina; Microbiology; Diagnostic tests/Investigation

Pablo Goldschmidt, Sandrine Degorge, Djida Benallaoua, Elena Basli, Laurence Batellier, et al.. New test for the diagnosis of bacterial endophthalmitis. British Journal of Ophthalmology, 2009, 93 (8), pp.1089-n/a. ⟨10.1136/bjo.2008.152181⟩. ⟨hal-00477830⟩