Gold/Silica biochips: applications to Surface Plasmon Resonance and fluorescence quenching

Thomas Mangeat (1) , Alexandre Berthier (2) , Céline Elie-Caille (1) , Maud Perrin (1) , Wilfrid Boireau (1) , Christian Pieralli (1) , Bruno Wacogne (1)

1 FEMTO-ST - Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174)
2 E2SNC / ESTROGENES - Estrogènes, Expression génique et pathologies du Système Nerveux Central - UFC

We report Gold/Silica biochips for low cost biosensor devices. Firstly, the study of biochemical interactions on silica by means of Surface Plasmon Resonance (SPR) is presented. Secondly, Gold/Silica biochips are employed to reduce the strong quenching that occurs when a fluorophore is close to the gold surface. Furthermore, the control of the Silica-like thickness allows optimizing the distance between the metallic surface and the fluorophore in order to enhance the fluorescent signal. These results represent the first steps towards highly sensitive, specific and low cost biosensors based, for example, on Surface Plasmon Coupled Emission (SPCE) techniques.

Domaines: Instrumentations et Détecteurs [physics.ins-det]

Thomas Mangeat, Alexandre Berthier, Céline Elie-Caille, Maud Perrin, Wilfrid Boireau, et al.. Gold/Silica biochips: applications to Surface Plasmon Resonance and fluorescence quenching. Laser Physics, 2009, 19 (2), pp.252-58. ⟨10.1134/S1054660X09020170⟩. ⟨hal-00446999⟩