Transgenesis and humanization of murine antibodies.

Michel Cogné (1, 2) , Sophie Duchez (1) , Virginie Pascal (1)

1 PMRIL - Physiologie Moléculaire de la Réponse Immune et des Lymphoproliférations
2 UNILIM - Université de Limoges

The properties of monoclonal antibodies explain why they are such a successful class of therapeutic molecules. However, pionneered initial antibodies were of murine origin and triggered an immune response which limited the therapeutic potential of the antibody and generated deleterious effects. Consequently, tremendous efforts have been developped to engineer these murine Ig by introducing human sequences in vitro, or in vivo by humanization of murine antibodies, leading to chimeric immunoglobulins, and more recently generation of fully human antibodies in transgenic mice with a more or less diversified V repertoire. These approaches have led to the development of an increasing number of these chimeric or humanized monoclonal antibodies entering pharmaceutical pipelines. double dagger.

Domaines: Immunologie

Michel Cogné, Sophie Duchez, Virginie Pascal. Transgenesis and humanization of murine antibodies.. Médecine/Sciences, 2009, 25 (12), pp.1149-54. ⟨hal-00443792⟩