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| HAL : hal-00442911, version 1 |
| PubMed : 19417908 |
| DOI : 10.1039/b819362b |
| Fiche détaillée |  Récupérer au format |
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| Lab on a Chip 9, 10 (2009) 1412-21 |
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| Microfluidic chips for the crystallization of biomacromolecules by counter-diffusion and on-chip crystal X-ray analysis. |
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Kaouthar Dhouib 1Chantal Khan Malek 2 |
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| (21/05/2009) |
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| Microfluidic devices were designed to perform on micromoles of biological macromolecules and viruses the search and the optimization of crystallization conditions by counter-diffusion, as well as the on-chip analysis of crystals by X-ray diffraction. Chips composed of microchannels were fabricated in poly-dimethylsiloxane (PDMS), poly-methyl-methacrylate (PMMA) and cyclo-olefin-copolymer (COC) by three distinct methods, namely replica casting, laser ablation and hot embossing. The geometry of the channels was chosen to ensure that crystallization occurs in a convection-free environment. The transparency of the materials is compatible with crystal growth monitoring by optical microscopy. The quality of the protein 3D structures derived from on-chip crystal analysis by X-ray diffraction using a synchrotron radiation was used to identify the most appropriate polymers. Altogether the results demonstrate that for a novel biomolecule, all steps from the initial search of crystallization conditions to X-ray diffraction data collection for 3D structure determination can be performed in a single chip. |
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| 1 : | Architecture et réactivité de l'ARN (ARN) |
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CNRS : UPR9002 – Université Louis Pasteur - Strasbourg I |
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| 2 : | Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (FEMTO-ST) |
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CNRS : UMR6174 – Université de Franche-Comté – Université de Technologie de Belfort-Montbeliard – Ecole Nationale Supérieure de Mécanique et des Microtechniques |
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| 3 : | Forschungszentrum Karlsruhe (FZK) |
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Helmoltz |
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| 4 : | Centre de Transfert des Micro et Nanotechnologies (CTMN) |
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CTMN |
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| 5 : | Institut des nanotechnologies de Lyon - Site d'Ecully (INL) |
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CNRS : UMR5270 – Université Claude Bernard - Lyon I – Institut National des Sciences Appliquées (INSA) - Lyon – Ecole Centrale de Lyon |
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| 6 : | Institut de biologie structurale (IBS) |
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CNRS : UMR5075 – CEA : DSV/IBS – Université Joseph Fourier - Grenoble I |
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| Domaine | : | Sciences du Vivant/Biochimie, Biologie Moléculaire/Biologie moléculaire Sciences du Vivant/Biologie cellulaire |
| hal-00442911, version 1 | |
| http://hal.archives-ouvertes.fr/hal-00442911 | |
| oai:hal.archives-ouvertes.fr:hal-00442911 | |
| Contributeur : Isabelle Martin | |
| Soumis le : Mercredi 23 Décembre 2009, 16:18:58 | |
| Dernière modification le : Mercredi 23 Décembre 2009, 16:18:58 | |
