Statistical assessment of variability of terminal restriction fragment length polymorphism analysis applied to complex microbial communities.

Pierre Rossi (1) , François Gillet (2, 3) , Emmanuelle Rohrbach (1) , Nouhou Diaby (1) , Christof Holliger (1)

1 Laboratory of Environmental Biotechnology
2 LCE - Laboratoire Chrono-environnement (UMR 6249)
3 ECOS - Laboratoire des systèmes écologiques

The variability of terminal restriction fragment polymorphism analysis applied to complex microbial communities was assessed statistically. Recent technological improvements were implemented in the successive steps of the procedure, resulting in a standardized procedure which provided a high level of reproducibility.

Domaines: Biodiversité et Ecologie; Microbiologie et Parasitologie

Pierre Rossi, François Gillet, Emmanuelle Rohrbach, Nouhou Diaby, Christof Holliger. Statistical assessment of variability of terminal restriction fragment length polymorphism analysis applied to complex microbial communities.. Applied and Environmental Microbiology, 2009, 75 (22), pp.7268-70. ⟨10.1128/AEM.00135-09⟩. ⟨hal-00434294⟩