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Soil Biology & Biochemistry 40 (2008) 1975-1979
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Variable selection in near infrared spectra for the biological characterization of soil and earthworm casts
Lauric Cécillon ( ) 1, Nathalie Cassagne 2, Sonia Czarnes 3, Raphaël Gros 4, Jean-Jacques Brun 1
(2008-07-01)

Near infrared reflectance spectroscopy (NIRS) was used to predict six biological properties of soil and earthworm casts including extracellular soil enzymes, microbial carbon, potential nitrification and denitrification. Partial least squares regression (PLSR) models were developed with a selection of the most important near infrared wavelengths. They reached coefficients of determination ranging from 0.81 to 0.91 and ratios of performance-to-deviation above 2.3. Variable selection with the variable importance in the projection (VIP) method increased dramatically the prediction performance of all models with an important contribution from the 1750–2500 nm region. We discuss whether selected wavelengths can be attributed to macronutrient availability or to microbial biomass. Wavelength selection in NIR spectra is recommended for improving PLSR models in soil research.
1:  Ecosystèmes montagnards (UR EMGR)
CEMAGREF
2:  Unité de Recherches Forestières Méditerranéennes (URFM)
INRA : UR0629
3:  Ecologie microbienne (EM)
CNRS : UMR5557 – INRA : UR1193 – Université Claude Bernard - Lyon I – Ecole Nationale Vétérinaire de Lyon
4:  Institut Méditerranéen d'Ecologie et de Paléoécologie (IMEP)
CNRS : UMR6116 – Université Paul Cézanne - Aix-Marseille III – Université de Provence - Aix-Marseille I – Université d'Avignon – IRD
Life Sciences/Agricultural sciences/Soil study
NIRS – PLSR – Wavelength selection – Soil biogenic structures – Soil macrofauna – Microbiological activity
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