%0 Conference Proceedings
%T Detection and light enhancement of glucose oxidase adsorbed on porous silicon microcavities
%+ Groupe d'étude des semiconducteurs (GES)
%A Palestino, Gabriela
%A Martin, Marta
%A Agarwal, Vivechana
%A Legros, Réné
%A Cloitre, Thierry
%A Zimanyi, Laszlo
%A Gergely, Csilla
%< avec comité de lecture
%@ 1610-1634
%J physica status solidi (c)
%( Physica Status Solidi C
%B 6th International Conference on Porous Semiconductor Science and Technology
%C Mallorca, Spain
%I Wiley
%V 6
%N 7
%P 1624 - 1628
%8 2008-03-10
%D 2008
%R 10.1002/pssc.200881006
%Z Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph]Conference papers
%X Porous silicon (PSi) structure is used as support material to detect protein infiltration and to induce fluorescence and second harmonic light enhancement from glucose oxidase (GOX). Functionalization and protein infiltration is monitored by specular reflectometry. Optical response enhancement of PSi microcavity structures compared to PSi single layers or Bragg mirrors is observed, when GOX is impregnated. Penetration of organic molecules along the PSi microcavity structure is demonstrated by energy dispersive X-ray profile. Enhanced fluorescence emission of GOX when adsorbed on PSi microcavity is evidenced by multi-photon microscopy (MPM). Second harmonic light generation is observed at some particular pores of PSi and subsequent resonance enhancement of the signal arising from the GOX adsorbed within the pores is detected. Our work evidences an improved device functionality of GOX-PSi microcavities due to strongly confined and localized light emission within these structures. This opens the way towards the application of PSi microcavity structures as amended biosensors based on their locally enhanced optical response. The second main achievement lies in the novelty of the used techniques. In contrast to the specular reflectometry used to monitor the macroscopic optical response of PSi structures, MPM presents a valuable alternative microscopic technique probing individual pores.
%G English
%Z Phoremost European Network of Excellence
%Z Project Nr. 511616: "NanoPhotonics to Realise Molecular Scale Technologies" and M06P01 Ecos-North French-Mexican program
%L hal-00393056
%U https://hal.science/hal-00393056
%~ CNRS
%~ UNIV-MONTP2
%~ GES
%~ UNIV-MONTPELLIER
%~ UM1-UM2