%0 Journal Article %T Three-dimensional spatial resolution of the nonlinear photoemission from biofunctionalized porous silicon microcavity %+ Groupe d'étude des semiconducteurs (GES) %A Martin, Marta %A Palestino, Gabriela %A Agarwal, Vivechana %A Cloitre, Thierry %A Zimanyi, Laszlo %A Gergely, Csilla %< avec comité de lecture %@ 0003-6951 %J Applied Physics Letters %I American Institute of Physics %V 94 %N 22 %P 223313 %8 2009-06-05 %D 2009 %R 10.1063/1.3148698 %Z Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph]Journal articles %X Infiltration of biomacromolecules into porous silicon photonic architectures results in biofunctionalized structures with unique properties. Characterization of their optical response and performance optimization in biomacromolecular detection and biophotonic application require a combination of optical and structural studies. Nonlinear optical microscopy is applied to study porous silicon microcavities with and without infiltrated glucose oxidase. The infiltrated protein acts as an internal two-photon-excited fluorescence emitter and second harmonic generator, enabling the in-depth visualization of the porous structure. Enhanced second harmonic generation and fluorescence emission by the porous silicon structure is experimentally associated with the defect layer. %G English %Z Phoremost European Network of Excellence %Z Project Nr. 511616: “NanoPhotonics to Realize Molecular Scale Technologies” and by the Micro and Nano-electronic Technological Center %Z University of Montpellier 2 %L hal-00391043 %U https://hal.science/hal-00391043 %~ CNRS %~ UNIV-MONTP2 %~ GES %~ UNIV-MONTPELLIER %~ UM1-UM2