Guidelines for the next 10 years of proteomics.

Marc R Wilkins (1) , Ron D Appel (2) , Jennifer E van Eyk (3) , Maxey C M Chung (4) , Angelika Görg (5) , Michael Hecker (6) , Lukas A Huber (7) , Hanno Langen (8) , Andrew J Link (9) , Young-Ki Paik (10) , Scott D Patterson (11) , Stephen R Pennington (12) , Thierry Rabilloud (13) , Richard J Simpson (14) , Walter Weiss (5) , Michael J Dunn (12)

1 Department of Biotechnology and Biomolecular Biosciences,
2 SIB/ISB - Swiss Institute of Bioinformatics
3 Departments of Medicine, Biological Chemistry and Biomedical Engineering,
4 Department of Biochemistry,
5 Department of Proteomics,
6 Institut fur Mikrobiologie,
7 Biocenter, Div. Cell Biology,
8 Roche Center for Medical Genomics,
9 Department of Microbiology and Immunology,
10 Yonsei Proteome Research Center and Department of Biochemistry,
11 AMGen - Applied Molecular Genetics
12 Proteome Research Centre, UCD Conway Institute for Biomolecular and Biomedical Research,
13 BBSI - Biochimie et biophysique des systèmes intégrés
14 Ludwig Institute for Cancer Research

In the last ten years, the field of proteomics has expanded at a rapid rate. A range of exciting new technology has been developed and enthusiastically applied to an enormous variety of biological questions. However, the degree of stringency required in proteomic data generation and analysis appears to have been underestimated. As a result, there are likely to be numerous published findings that are of questionable quality, requiring further confirmation and/or validation. This manuscript outlines a number of key issues in proteomic research, including those associated with experimental design, differential display and biomarker discovery, protein identification and analytical incompleteness. In an effort to set a standard that reflects current thinking on the necessary and desirable characteristics of publishable manuscripts in the field, a minimal set of guidelines for proteomics research is then described. These guidelines will serve as a set of criteria which editors of PROTEOMICS will use for assessment of future submissions to the Journal.

Domaines: Génomique, Transcriptomique et Protéomique [q-bio.GN]

Marc R Wilkins, Ron D Appel, Jennifer E van Eyk, Maxey C M Chung, Angelika Görg, et al.. Guidelines for the next 10 years of proteomics.. Proteomics, 2006, 6 (1), pp.4-8. ⟨10.1002/pmic.200500856⟩. ⟨hal-00373322⟩