HAL: hal-00370780, version 1

PubMed: 18502920

PubMed Central: 2493162

DOI: 10.1128/AEM.02851-07

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Appl Environ Microbiol 74, 14 (2008) 4567-73

Diversity surveys and evolutionary relationships of aoxB genes in aerobic arsenite-oxidizing bacteria.

Marianne Quéméneur, Audrey Heinrich-Salmeron 1, Daniel Muller 2, 3, 4, Didier Lièvremont 1, Michel Jauzein, Philippe N Bertin 1, Francis Garrido 5, Catherine Joulian 5

(2008-07)

A new primer set was designed to specifically amplify ca. 1,100 bp of aoxB genes encoding the As(III) oxidase catalytic subunit from taxonomically diverse aerobic As(III)-oxidizing bacteria. Comparative analysis of AoxB protein sequences showed variable conservation levels and highlighted the conservation of essential amino acids and structural motifs. AoxB phylogeny of pure strains showed well-discriminated taxonomic groups and was similar to 16S rRNA phylogeny. Alphaproteobacteria-, Betaproteobacteria-, and Gammaproteobacteria-related sequences were retrieved from environmental surveys, demonstrating their prevalence in mesophilic As-contaminated soils. Our study underlines the usefulness of the aoxB gene as a functional marker of aerobic As(III) oxidizers.

1:	Génétique moléculaire, génomique, microbiologie (GMGM)
	CNRS : UMR7156 – Université Louis Pasteur - Strasbourg I
2:	Ecologie microbienne (EM)
	CNRS : UMR5557 – INRA : UR1193 – Université Claude Bernard - Lyon I – Ecole Nationale Vétérinaire de Lyon
3:	Laboratoire de Biologie Tumorale
	CRLCC Paul Strauss
4:	XLIM (XLIM)
	CNRS : UMR6172 – Université de Limoges
5:	Bureau de recherches géologiques et minières (BRGM)
	BRGM

Subject

:

Life Sciences/Biochemistry, Molecular Biology

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From: Nathalie De Hoest <>

Submitted on: Wednesday, 25 March 2009 12:19:57

Updated on: Thursday, 26 March 2009 08:34:32