| HAL : hal-00370780, version 1 |
| PubMed : 18502920 |
| PubMed Central : 2493162 |
| DOI : 10.1128/AEM.02851-07 |
| Fiche détaillée | Récupérer au format |
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| Appl Environ Microbiol 74, 14 (2008) 4567-73 |
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| Diversity surveys and evolutionary relationships of aoxB genes in aerobic arsenite-oxidizing bacteria. |
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| Marianne QuéméneurAudrey Heinrich-Salmeron 1 |
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| (07/2008) |
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| A new primer set was designed to specifically amplify ca. 1,100 bp of aoxB genes encoding the As(III) oxidase catalytic subunit from taxonomically diverse aerobic As(III)-oxidizing bacteria. Comparative analysis of AoxB protein sequences showed variable conservation levels and highlighted the conservation of essential amino acids and structural motifs. AoxB phylogeny of pure strains showed well-discriminated taxonomic groups and was similar to 16S rRNA phylogeny. Alphaproteobacteria-, Betaproteobacteria-, and Gammaproteobacteria-related sequences were retrieved from environmental surveys, demonstrating their prevalence in mesophilic As-contaminated soils. Our study underlines the usefulness of the aoxB gene as a functional marker of aerobic As(III) oxidizers. |
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| 1 : | Génétique moléculaire, génomique, microbiologie (GMGM) |
| CNRS : UMR7156 – Université Louis Pasteur - Strasbourg I | |
| 2 : | Ecologie microbienne (EM) |
| CNRS : UMR5557 – INRA : UR1193 – Université Claude Bernard - Lyon I – Ecole Nationale Vétérinaire de Lyon | |
| 3 : | Laboratoire de Biologie Tumorale |
| CRLCC Paul Strauss | |
| 4 : | XLIM (XLIM) |
| CNRS : UMR6172 – Université de Limoges | |
| 5 : | Bureau de recherches géologiques et minières (BRGM) |
| BRGM | |
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| Domaine | : | Sciences du Vivant/Biochimie, Biologie Moléculaire |
| hal-00370780, version 1 | |
| http://hal.archives-ouvertes.fr/hal-00370780 | |
| oai:hal.archives-ouvertes.fr:hal-00370780 | |
| Contributeur : Nathalie De Hoest | |
| Soumis le : Mercredi 25 Mars 2009, 12:19:57 | |
| Dernière modification le : Jeudi 26 Mars 2009, 08:34:32 | |