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| HAL : hal-00346660, version 1 |
| PubMed : 19064255 |
| DOI : 10.1016/j.chom.2008.11.002 |
| Fiche détaillée |  Récupérer au format |
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| Cell Host & Microbe 4, 6 (2008) 543-54 |
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| TLR-independent type I interferon induction in response to an extracellular bacterial pathogen via intracellular recognition of its DNA. |
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| Marie Charrel-Dennis 1Eicke Latz 1 |
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| (11/12/2008) |
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| Type I interferon (IFN) is an important host defense cytokine against intracellular pathogens, mainly viruses. In assessing IFN production in response to group B streptococcus (GBS), we find that IFN-beta was produced by macrophages upon stimulation with both heat-killed and live GBS. Exposure of macrophages to heat-killed GBS activated a Toll-like receptor (TLR)-dependent pathway, whereas live GBS activated a TLR/NOD/RIG-like receptor (RLR)-independent pathway. This latter pathway required bacterial phagocytosis, proteolytic bacterial degradation, and phagolysosomal membrane destruction by GBS pore-forming toxins, leading to the release of bacterial DNA into the cytosol. GBS DNA in the cytosol induced IFN-beta production via a pathway dependent on the activation of the serine-threonine kinase TBK1 and phosphorylation of the transcription factor IRF3. Thus, activation of IFN-alpha/-beta production during infection with GBS, commonly considered an extracellular pathogen, appears to result from the interaction of GBS DNA with a putative intracellular DNA sensor or receptor. |
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| 1 : | Division of Infectious Diseases and Immunology |
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University of Massachusetts Medical School |
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| 2 : | Biologie des Bactéries Pathogènes à Gram-positif |
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Institut Pasteur de Paris – CNRS : URA2172 |
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| 3 : | Channing Laboratory |
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Brigham and Women's Hospital - Harvard Medical School |
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| 4 : | Department of Microbiology and Molecular Genetics |
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Harvard Medical School |
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| Domaine | : | Sciences du Vivant/Microbiologie et Parasitologie |
| hal-00346660, version 1 | |
| http://hal.archives-ouvertes.fr/hal-00346660 | |
| oai:hal.archives-ouvertes.fr:hal-00346660 | |
| Contributeur : Christine Dugast | |
| Soumis le : Vendredi 12 Décembre 2008, 09:56:59 | |
| Dernière modification le : Vendredi 26 Décembre 2008, 18:09:16 | |
