Phosphonate monolayers functionalized by silver thiolate species as antibacterial nanocoatings on titanium and stainless steel

Julien Amalric (1) , P. Hubert Mutin (1) , Gilles Guerrero (1) , Arnaud Ponche (2) , Albert Sotto (3) , Jean-Philippe Lavigne (3, 4)

1 ICGM ICMMM - Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier
2 ICSI - Institut de Chimie et des Surfaces et Interfaces
3 Systèmes de sécrétion de type IV et virulence bactérienne
4 Laboratoire de bactériologie

Titanium and stainless steel substrates were modified by grafting with mercaptododecyl phosphonic acid (MDPA) followed by reaction with silver nitrate (AgNO3), in order to investigate the potential of phosphonate self-assembled monolayers functionalized by silver thiolate species as antibacterial nanocoatings for inorganic biomaterials. The samples were characterized by Fourier transform infrared (FTIR) spectroscopy in grazing-incidence mode, water contact angle measurements, and X-ray photoelectron spectroscopy (XPS). The influence of the surface modification on bacterial adhesion and biofilm growth was investigated in vitro using E. coli, P. aeruginosa, S. epidermidis, and S. aureus strains. The stability of the the monolayer in blood-mimicking medium was examined. Despite their very low silver content, MDPA+AgNO3 monolayers strongly decreased bacterial adhesion (>99.9% reduction in the number of viable adherent bacteria) and biofilm formation in comparison to the bare substrates.

Domaines: Matériaux

Julien Amalric, P. Hubert Mutin, Gilles Guerrero, Arnaud Ponche, Albert Sotto, et al.. Phosphonate monolayers functionalized by silver thiolate species as antibacterial nanocoatings on titanium and stainless steel. Journal of Materials Chemistry, 2009, 19 (1), pp.141-149. ⟨10.1039/b813344a⟩. ⟨hal-00332902⟩