System identification of the intracellular photoreaction process induced by photodynamic therapy

Simona Dobre (1) , Thierry Bastogne (1) , Muriel Barberi-Heyob (1) , Denise Bechet (1) , Jacques Didelon (1) , Alain Richard (1)

1 CRAN - Centre de Recherche en Automatique de Nancy

Photodynamic therapy (PDT) is an alternative treatment for cancer that involves the administration of a photosensitizing agent, which is activated by light at a specific wavelength. This illumination causes a sequence of photoreactions, which - in the presence of molecular oxygen - is supposed to be responsible for the death of the tumor cells. The PDT efficiency stems from the optimal interaction between these three factors (light, drug and oxygen). In this paper, a new approach is proposed to estimate photophysical parameters which characterize the ability of a photosensitizing drug to produce singlet oxygen. This approach is based on system identification techniques. This model-based method would allow biologists to estimate all the photophysical parameters from spectro-fluorescence data generated by only one experiment. Secondly, contrary to usual techniques which are restricted to in vitro studies, this approach can be directly applied to in vivo data.

Domaines: Automatique / Robotique

Simona Dobre, Thierry Bastogne, Muriel Barberi-Heyob, Denise Bechet, Jacques Didelon, et al.. System identification of the intracellular photoreaction process induced by photodynamic therapy. 16th Mediterranean Conference on Control and Automation, MED'08, Jun 2008, Ajaccio, France. pp.1729-1734, ⟨10.1109/MED.2008.4602185⟩. ⟨hal-00322893⟩