DISSECTING INDIVIDUAL LIGAND-RECEPTOR BONDS WITH A LAMINAR FLOW CHAMBER

Anne Pierres (1) , Joana Vitte (1) , Anne-Marie Benoliel (1) , Pierre Bongrand (1)

1 LAI - Adhésion et Inflammation

The most important function of proteins may well be to bind to other biomolecules. It has long been felt that kinetic rates of bond formation and dissociation between soluble receptors and ligands might account for most features of the binding process. Only theoretical considerations allowed to predict the behaviour of surface-attached receptors from the properties of soluble forms. During the last decade, experimental progress essentially based on flow chambers, atomic force microscopes or biomembrane force probes allowed direct analysis of biomolecule interaction at the single bond level and gave new insight into previously ignored features such as bond mechanical properties or energy landscapes. The aim of this review is (i) to describe the main advances brought by laminar flow chambers, including information on bond response to forces, multiplicity of binding states, kinetics of bond formation between attached structures, effect of molecular environment on receptor efficiency and behaviour of multivalent attachment, (ii) to compare results obtain by this and other techniques on a few well defined molecular systems, and (iii) to discuss the limitations of the flow chamber method. It is concluded that a new framework may be needed to account for the effective behaviour of biomolecule association.

Mots clés: on-rate; off-rate; bond strength; intermediate state

Domaines: Biophysique [physics.bio-ph]; Biophysique

Anne Pierres, Joana Vitte, Anne-Marie Benoliel, Pierre Bongrand. DISSECTING INDIVIDUAL LIGAND-RECEPTOR BONDS WITH A LAMINAR FLOW CHAMBER: DISSECTING INDIVIDUAL LIGAND-RECEPTOR BONDS WITH A LAMINAR FLOW CHAMBER. Biophysical Reviews and Letters, 2006, 1, pp.231-257. ⟨hal-00320210⟩