HAL :: [hal-00312918, version 1] Strategy for in situ detection of natural transformation-based horizontal gene transfer events

HAL : hal-00312918, version 1

DOI : 10.1128/AEM.02185-07

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Applied and Environmental Microbiology 74, 4 (2008) 1250-1254

Strategy for in situ detection of natural transformation-based horizontal gene transfer events

Aurora Rizzi, Alessandra Pontiroli ¹, Lorenzo Brusetti, Sara Borin, Claudia Sorlini, Alessandro Abruzzese, Gian Attilio Sacchi ², Timothy M. Vogel ^1, 3, Pascal Simonet (

) ^1, 3, Marco Bazzicalupo, Kaare Magne Nielsen, Jean-Michel Monier ^1, 3, Daniele Daffonchio ⁴

(02/2008)

A strategy is described that enables the in situ detection of natural transformation in Acinetobacter baylyi BD413 by the expression of a green fluorescent protein. Microscale detection of bacterial transformants growing on plant tissues was shown by fluorescence microscopy and indicated that cultivation-based selection of transformants on antibiotic-containing agar plates underestimates transformation frequencies.

1 :	Ampère

	CNRS : UMR5005 – Université Claude Bernard - Lyon I – Institut National des Sciences Appliquées de Lyon – Ecole Centrale de Lyon

2 :	Dipartimento di Produzione Vegetale

	Università degli studi di Milano

3 :	Ecologie microbienne (EM)

	CNRS : UMR5557 – INRA : UR1193 – Université Claude Bernard - Lyon I – Ecole Nationale Vétérinaire de Lyon

4 :	CONISMA ULR (CONISMA)

	Università degli studi di Milano

Domaine

Sciences de l'ingénieur/Energie électrique

Mots Clés : ACINETOBACTER SP – TRANSGENIC PLANTS – STRAIN BD413 – DNA – BACTERIA – MICROORGANISMS – INNOVATION – EVOLUTION


hal-00312918, version 1
http://hal.archives-ouvertes.fr/hal-00312918
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Soumis le : Mardi 26 Août 2008, 23:28:38
Dernière modification le : Mardi 17 Mars 2009, 14:41:48