Quantification of the CBD-FITC conjugates surface coating on cellulose fibres - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue BMC Biotechnology Année : 2008

Quantification of the CBD-FITC conjugates surface coating on cellulose fibres

Résumé

Background: Cellulose Binding Domains (CBD) were conjugated with fluorescein isothiocyanate (FITC). The surface concentration of the Binding Domains adsorbed on cellulose fibres was determined by fluorescence image analysis. Results: For a CBD-FITC concentration of 60 mg/L, a coating fraction of 78% and 110% was estimated for Portucel and Whatman fibres, respectively. For a saturating CBD concentration, using Whatman CF11 fibres, a surface concentration of 25.2 × 10-13 mol/mm2 was estimated, the equivalent to 4 protein monolayers. This result does not imply the existence of several adsorbed protein layers. Conclusion: It was verified that CBDs were able to penetrate the fibres, according to confocal microscopy and TEM-immunolabelling analysis. The surface concentration of adsorbed CBDs was greater on amorphous fibres (phosphoric acid swollen) than on more crystalline ones (Whatman CF11 and Sigmacell 20).

Domaines

Polymères
Fichier principal
Vignette du fichier
P08-13.pdf (1.13 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-00303871 , version 1 (06-08-2008)

Identifiants

  • HAL Id : hal-00303871 , version 1

Citer

R. Pinto, A.L. Amaral, E.C. Ferreira, M. Mota, M. Vilanova, et al.. Quantification of the CBD-FITC conjugates surface coating on cellulose fibres. BMC Biotechnology, 2008, pp.1-7. ⟨hal-00303871⟩

Collections

UGA CNRS CERMAV
92 Consultations
201 Téléchargements

Partager

Gmail Facebook X LinkedIn More