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| HAL : hal-00282468, version 1 |
| PubMed : 18256234 |
| PubMed Central : 2279246 |
| DOI : 10.1101/gr.074534.107 |
| Fiche détaillée |  Récupérer au format |
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| Genome Researchearch 18, 4 (2008) 585-96 |
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| Analysis of sequence variability in the macronuclear DNA of Paramecium tetraurelia: a somatic view of the germline. |
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| Laurent Duret 1Jean Cohen 2, 3, 4, 5, 6, 7, 8, 9 |
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| (04/2008) |
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| Ciliates are the only unicellular eukaryotes known to separate germinal and somatic functions. Diploid but silent micronuclei transmit the genetic information to the next sexual generation. Polyploid macronuclei express the genetic information from a streamlined version of the genome but are replaced at each sexual generation. The macronuclear genome of Paramecium tetraurelia was recently sequenced by a shotgun approach, providing access to the gene repertoire. The 72-Mb assembly represents a consensus sequence for the somatic DNA, which is produced after sexual events by reproducible rearrangements of the zygotic genome involving elimination of repeated sequences, precise excision of unique-copy internal eliminated sequences (IES), and amplification of the cellular genes to high copy number. We report use of the shotgun sequencing data (>10(6) reads representing 13 x coverage of a completely homozygous clone) to evaluate variability in the somatic DNA produced by these developmental genome rearrangements. Although DNA amplification appears uniform, both of the DNA elimination processes produce sequence heterogeneity. The variability that arises from IES excision allowed identification of hundreds of putative new IESs, compared to 42 that were previously known, and revealed cases of erroneous excision of segments of coding sequences. We demonstrate that IESs in coding regions are under selective pressure to introduce premature termination of translation in case of excision failure. |
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| 1 : | Laboratoire de Biométrie et Biologie Evolutive (LBBE) |
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Université Claude Bernard - Lyon I – CNRS : UMR5558 – INRIA |
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| 2 : | Department of Psychology (PRINCETON UNIVERSITY) |
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Princeton University |
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| 3 : | Laboratoire de Mécanique et Ingénieries (LAMI) |
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Université Blaise Pascal - Clermont-Ferrand II – Institut Français de Mécanique Avancée |
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| 4 : | Institut d'Informatique et de Mathématiques Appliquées de Grenoble (IMAG) |
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CNRS – Institut National Polytechnique de Grenoble (INPG) – Université Joseph Fourier - Grenoble I |
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| 5 : | Laboratoire d'Algorithmique Complexité et Logique (LACL) |
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CNRS : FRE2673 – Université Paris-Est Créteil Val-de-Marne (UPEC) |
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| 6 : | Institute of Atomic Physics |
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Université de Roumanie |
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| 7 : | Architecture, Urbanisme, Sociétés (AUS) |
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CNRS : UMR7136 – Université Paris VIII - Vincennes Saint-Denis – Université Paris X - Paris Ouest Nanterre La Défense – Université Paris-Est Créteil Val-de-Marne (UPEC) – Ecole d'architecture Paris-Malaquais – Ecole d'architecture Paris-Belleville |
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| 8 : | Coordination nationale des GROG |
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open Rome |
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| 9 : | Centre de génétique moléculaire (CGM) |
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CNRS : UPR2167 |
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| 10 : | Structure et évolution des génomes (SEG) |
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CNRS : UMR8030 – CNS – Université d'Evry-Val d'Essonne |
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| 11 : | Génomique métabolique (UMR 8030) |
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CNRS : UMR8030 – CEA : DSV/IG – Université d'Evry-Val d'Essonne |
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| 12 : | Génomes et cancer (GC) |
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CNRS : FRE2939 – Institut Gustave Roussy – Université Paris XI - Paris Sud |
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| 13 : | Laboratoire de Génétique Oncologique |
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CNRS : FRE2939 – Institut Gustave Roussy |
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| 14 : | Enseignement-Formation-Apprentissage (EFA) |
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Aucune |
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| 15 : | Régulation de l'expression génétique (REG) |
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CNRS : UMR8541 – Ecole normale supérieure de Paris - ENS Paris |
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| 16 : | Institute of Physics (NCCR) |
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University of Basel |
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| 17 : | Modèles et simulation pour l'architecture, l'urbanisme et le paysage (MAP) |
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EC ARCHIT MARSEILLE – EC ARCHIT NANCY – EC ARCHIT LYON – EC ARCHIT TOULOUSE – CNRS : UMR694 – Institut National des Sciences Appliquées (INSA) - Strasbourg – Ministère de la culture |
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| Domaine | : | Sciences du Vivant/Cancer |
| hal-00282468, version 1 | |
| http://hal.archives-ouvertes.fr/hal-00282468 | |
| oai:hal.archives-ouvertes.fr:hal-00282468 | |
| Contributeur : Christelle Bouchot-Vergnenegre | |
| Soumis le : Mardi 27 Mai 2008, 15:53:23 | |
| Dernière modification le : Mercredi 4 Juin 2008, 17:19:34 | |
