Chemoenzymatic synthesis of L-tyrosine derivative for a transketolase assay

Franck Charmantray (1) , Virgil Hélaine (1) , Angelika Lasikova (2) , Bertrand Legeret (1) , Laurence Hecquet (1)

1 SEESIB - Synthèse et étude de systèmes à intêret biologique
2 Department of Organic Chemistry

We have prepared an l-tyrosine derivative bearing a d-threo ketose moiety by a convenient chemoenzymatic route. This compound is of potential interest for developing stereospecific assays for enzymes catalyzing C–C bond cleavage such as transketolase. We showed in vitro by analytical studies (LC/MS and 31P NMR) that this compound can release l-tyrosine in the presence of wild type TK extract and bovine serum albumin. This assay is the first step towards a mutant TK selection test that could be developed for yeast cells auxotrophic for l-tyrosine

Domaines: Catalyse

Franck Charmantray, Virgil Hélaine, Angelika Lasikova, Bertrand Legeret, Laurence Hecquet. Chemoenzymatic synthesis of L-tyrosine derivative for a transketolase assay. Tetrahedron Letters, 2008, 49, pp.3229-3233. ⟨hal-00275587⟩