ELECTROCATALYTICAL MAGNETOBIOSENSING BASED ON GOLD NANOPARTICLES

Alfredo de La Escosura Muniz (1) , Marisa Maltez da Costa (1) , M. Teresa Castaneda Briones (1) , Salvador Alegret (2) , Arben Merkoci (1)

1 Nanobioelectronics and Biosensors Group
2 Group of Sensors and Biosensors

In this work, electrocatalytical methods are developed in order to improve the sensitivity of model biosensors based on a labelling method using AuNPs. Microparamagnetic beads (MB) are used as primary biological molecules immobilisation platforms and AuNPs modified with secondary biological molecules as high sensitive electrochemical labels. The carbon electrodes, used as transducer, incorporate a magnet that allows the collection / immobilization on its surface of the biological sandwich attached to the MB. The final detection is based on the catalytic effect of AuNPs on the electroreduction of different species, like silver ions. The low levels of AuNPs detected with these methods allow the obtaining of biosensors with low protein and ss-DNA target detection limits, with special interest for further applications in clinical analysis, food quality and safety as well as other industrial applications.

Domaines: Science des matériaux [cond-mat.mtrl-sci]

Alfredo de La Escosura Muniz, Marisa Maltez da Costa, M. Teresa Castaneda Briones, Salvador Alegret, Arben Merkoci. ELECTROCATALYTICAL MAGNETOBIOSENSING BASED ON GOLD NANOPARTICLES. ENS 2007, Dec 2007, Paris, France. pp.45-48. ⟨hal-00202510⟩