RNAi screen in Drosophila cells reveals the involvement of the Tom complex in Chlamydia infection.

Isabelle Derré (1) , Marc Pypaert (2) , Alice Dautry-Varsat (3) , Hervé Agaisse (2)

1 Section of Microbial Pathogenesis
2 Department of Cell Biology [New Haven]
3 Biologie des Interactions Cellulaires

Chlamydia spp. are intracellular obligate bacterial pathogens that infect a wide range of host cells. Here, we show that C. caviae enters, replicates, and performs a complete developmental cycle in Drosophila SL2 cells. Using this model system, we have performed a genome-wide RNA interference screen and identified 54 factors that, when depleted, inhibit C. caviae infection. By testing the effect of each candidate's knock down on L. monocytogenes infection, we have identified 31 candidates presumably specific of C. caviae infection. We found factors expected to have an effect on Chlamydia infection, such as heparansulfate glycosaminoglycans and actin and microtubule remodeling factors. We also identified factors that were not previously described as involved in Chlamydia infection. For instance, we identified members of the Tim-Tom complex, a multiprotein complex involved in the recognition and import of nuclear-encoded proteins to the mitochondria, as required for C. caviae infection of Drosophila cells. Finally, we confirmed that depletion of either Tom40 or Tom22 also reduced C. caviae infection in mammalian cells. However, C. trachomatis infection was not affected, suggesting that the mechanism involved is C. caviae specific.

Domaines: Biologie moléculaire

Isabelle Derré, Marc Pypaert, Alice Dautry-Varsat, Hervé Agaisse. RNAi screen in Drosophila cells reveals the involvement of the Tom complex in Chlamydia infection.. PLoS Pathogens, 2007, 3 (10), pp.1446-58. ⟨10.1371/journal.ppat.0030155⟩. ⟨hal-00185686⟩