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Article Dans Une Revue Nature Année : 2006

RNA-mediated non-mendelian inheritance of an epigenetic change in the mouse.

Résumé

Paramutation is a heritable epigenetic modification induced in plants by cross-talk between allelic loci. Here we report a similar modification of the mouse Kit gene in the progeny of heterozygotes with the null mutant Kit(tm1Alf) (a lacZ insertion). In spite of a homozygous wild-type genotype, their offspring maintain, to a variable extent, the white spots characteristic of Kit mutant animals. Efficiently inherited from either male or female parents, the modified phenotype results from a decrease in Kit messenger RNA levels with the accumulation of non-polyadenylated RNA molecules of abnormal sizes. Sustained transcriptional activity at the postmeiotic stages--at which time the gene is normally silent--leads to the accumulation of RNA in spermatozoa. Microinjection into fertilized eggs either of total RNA from Kit(tm1Alf/+) heterozygotes or of Kit-specific microRNAs induced a heritable white tail phenotype. Our results identify an unexpected mode of epigenetic inheritance associated with the zygotic transfer of RNA molecules.
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Dates et versions

hal-00166237 , version 1 (03-08-2007)

Identifiants

Citer

Minoo Rassoulzadegan, Valérie Grandjean, Pierre Gounon, Stéphane Vincent, Isabelle Gillot, et al.. RNA-mediated non-mendelian inheritance of an epigenetic change in the mouse.. Nature, 2006, 441 (7092), pp.469-74. ⟨10.1038/nature04674⟩. ⟨hal-00166237⟩
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