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| HAL : hal-00140691, version 1 |
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| FEMS microbiology letters 257, 2 (2006) 243-252 |
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| Plasmid-encoded gamma-hexachlorocyclohexane degradation genes and insertion sequences in Sphingobium francense (ex-Sphingomonas paucimobilis Sp+) |
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| Hélène Cérémonie 1, 2Hasna Boubakri |
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| (04/2006) |
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| The lin genes encode the gamma-hexachlorocyclohexane (gamma-HCH or lindane) catabolic pathway in lindane-degrading strains. The location and stability of these genes have been explored in the lindane-degrading Sphingobium francense strain Sp+, and in two nonlindane-degrading mutants (Sp1- and Sp2-). The lin genes, linA, linB, linE and linX were localized by hybridization on three of the six plasmids of the S. francense strain Sp+ showing dispersal within the genome. The linC gene was detected by PCR, but was not detected by hybridization on any of the plasmids. The hybridization of the linA and linX genes was negative with the two nonlindane-degrading mutants S. francense strains, Sp1- and Sp2-. The dynamic of this genome associated with gene loss and acquisition, and plasmid rearrangement was explored by a search for associated insertion sequences. A new insertion sequence, ISSppa4, belonging to the IS21 family was detected and compared with IS6100 and ISsp1. Insertion sequence localization was explored on different hybridization patterns (plasmid, total genome) with the lindane-degrading Sp+ strain and the two nondegrading derivatives (Sp1-, Sp2-). Insertion sequence movement and plasmid rearrangement could explain the emergence of the nonlindane-degrading mutants. |
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| 1 : | Ampère |
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CNRS : UMR5005 – Université Claude Bernard - Lyon I – Institut National des Sciences Appliquées de Lyon – Ecole Centrale de Lyon |
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| 2 : | Centre de génie électrique de Lyon (CEGELY) |
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CNRS : UMR5005 – Université Claude Bernard - Lyon I – Institut National des Sciences Appliquées de Lyon – Ecole Centrale de Lyon |
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| 3 : | Ecologie microbienne (EM) |
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CNRS : UMR5557 – INRA : UR1193 – Université Claude Bernard - Lyon I – Ecole Nationale Vétérinaire de Lyon |
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| Domaine | : | Sciences de l'ingénieur/Energie électrique |
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| lindane – adaptation – plasmid – Sphingobium francense – insertion sequences – genetic instability – PSEUDOMONAS-PAUCIMOBILIS – DEHALOGENASE GENE – DEGRADING BACTERIUM – LIN GENES – CATABOLIC GENES – CLONING – IDENTIFICATION – STRAINS – GENOME – ORGANIZATION |
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| hal-00140691, version 1 | |
| http://hal.archives-ouvertes.fr/hal-00140691 | |
| oai:hal.archives-ouvertes.fr:hal-00140691 | |
| Contributeur : Publications Ampère | |
| Soumis le : Lundi 9 Avril 2007, 23:02:23 | |
| Dernière modification le : Jeudi 12 Avril 2007, 16:29:44 | |
