 |
|
|
|
| HAL : hal-00135745, version 1 |
| DOI : 10.1111/j.1462-2920.2006.01087.x |
| Fiche détaillée |  Récupérer au format |
|
|
| Environmental microbiology 8, 12 (2006) 2106-2114 |
|
|
|
|
| Presence of Hydrogenophilus thermoluteolus DNA in accretion ice in the subglacial Lake Vostok, Antarctica, assessed using rrs, cbb and hox |
|
|
| Céline Lavire 1P. Normand 1 |
|
|
| (2006) |
|
|
|
| The 3561 m Vostok ice core sample originating from the subglacial Lake Vostok accretion (frozen lake water) ice with sediment inclusions was thoroughly studied by various means to confirm the presence of the thermophile bacterium Hydrogenophilus thermoluteolus reported earlier in the 3607 m accretion ice sample. PCR and molecular-phylogenetic analyses performed in two independent laboratories were made using different 16S rRNA gene ( rrs ) targeted primers. As a result, rrs -targeted PCR permitted to recover several very closely related clones with a small genetic distance to Hydrogenophilus thermoluteolus ( < 1%). In addition, RubisCO ( cbbL or rbcL ) and NiFe-Hydrogenase (hoxV or hupL ) targeted PCR have also allowed to recover sequences highly related to Hydrogenophilus thermoluteolus . All these results point to the presence of thermophilic chemoautotrophic microorganisms in Lake Vostok accretion ice. They presumably originate from deep faults in the bedrock cavity containing the lake in which episodes of seismotectonic activity would release debris along with microbial cells. |
|
|
|
|
|
|
|
|
|
|
|
| 1 : | Ecologie microbienne (EM) |
|
|
|
CNRS : UMR5557 – INRA : UR1193 – Université Claude Bernard - Lyon I – Ecole Nationale Vétérinaire de Lyon |
|
|
| 2 : | Saint Petersburg Nuclear Physics Institute RAS |
|
|
|
St Petersburg Nuclear Physics Institute |
|
|
| 3 : | Laboratoire de glaciologie et géophysique de l'environnement (LGGE) |
|
|
|
CNRS : UMR5183 – OSUG – INSU – Université Joseph Fourier - Grenoble I |
|
|
| 4 : | Laboratoire de microbiologie des environnements extrêmophiles (LM2E) |
|
|
|
CNRS : UMR6197 – Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) – Université de Bretagne Occidentale - Brest |
|
|
| 5 : | Centre de génétique moléculaire et cellulaire (CGMC) |
|
|
|
CNRS : UMR5534 – Université Claude Bernard - Lyon I |
|
|
|
|
|
|
|
|
|
| 1 |
|
|
|
|
| Domaine | : | Sciences du Vivant/Microbiologie et Parasitologie |
| hal-00135745, version 1 | |
| http://hal.archives-ouvertes.fr/hal-00135745 | |
| oai:hal.archives-ouvertes.fr:hal-00135745 | |
| Contributeur : Christine Delorme | |
| Soumis le : Jeudi 8 Mars 2007, 16:13:55 | |
| Dernière modification le : Jeudi 8 Mars 2007, 16:13:55 | |
