Preliminary X-ray investigations of several crystal forms of the ferripyoverdine FpvA outer membrane receptor from Pseudomonas aeruginosa bound to ferripyoverdine

C. Wirth , F. Hoegy , F. Pattus (1) , D. Cobessi

1 Institut Gilbert-Laustriat : Biomolécules, Biotechnologie, Innovation Thérapeutique

Ferripyoverdine transport across the outer membrane of Pseudomonas aeruginosa by the pyoverdine receptor FpvA and the transcriptional regulation of FpvA involve interactions of the FpvA N-terminal TonB box and signalling domain with proteins from the inner membrane. Several crystallization conditions of FpvA-Pvd-Fe solubilized in C8E4 detergent were obtained and X-ray data were collected from three crystal forms. The resolution limits range from 3.15 to 2.7 angstroms depending on the crystal form. From preliminary analysis of the electron-density maps, the first full-length structure of an outer membrane receptor including a signalling domain should be determined.

Mots clés: Bacterial Outer Membrane Proteins/*chemistry/metabolism; Crystallization; Crystallography; X-Ray; Oligopeptides/*metabolism; Pseudomonas aeruginosa/chemistry; Research Support; Non-U.S. Gov't

C. Wirth, F. Hoegy, F. Pattus, D. Cobessi. Preliminary X-ray investigations of several crystal forms of the ferripyoverdine FpvA outer membrane receptor from Pseudomonas aeruginosa bound to ferripyoverdine. Acta Crystallograph Sect F Struct Biol Cryst Commun, 2006, 62, pp.460-3. ⟨hal-00130012⟩