| Urine deposition on grassland causes significant N2O losses, which in some cases may result from increased denitrification stimulated by labile compounds released from scorched plant roots. Two 12-day experiments were conducted in 13Clabelled grassland monoliths to investigate the link between N2O production and carbon mineralization following application of low rates of urine-N. Measurements of N2O and CO2 emissions from the monoliths as well as d13C signal of evolved CO2 were done on day )4, )1, 0, 1, 2, 4, 5, 6 and 7 after application of urine corresponding to 3.1 and 5.5 g N m)2 in the first and second experiment, respectively. The d13C signal was also determined for soil organic matter, dissolved organic C and CO2 evolved by microbial respiration. In addition, denitrifying enzyme activity (DEA) and nitrifying enzyme activity (NEA) were measured on day )1, 2 and 7 after the first urine application event. Urine did not affect DEA, whereas NEA was enhanced 2 days after urine application. In the first experiment, urine had no significant effect on the N2O flux, which was generally low ()8 to 14lg N2O-N m)2 h)1). After the second application event, the N2O emission increased significantly to 87 lg N2O-N m)2 h)1 and the N2O emission factor for the added urine-N was 0.18%. However, the associated 13C signal of soil respiration was unaffected by urine. Consequently, the increased N2O emission from the simulated low N-urine patches was not caused by enhanced denitrification stimulated by labile compounds released from scorched plant roots. |
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