Natural polymorphism of cytomegalovirus DNA polymerase lies in two nonconserved regions located between domains delta-C and II and between domains III and I.

L. Auray (1) , S. Alain (2) , K. Gourlain (1) , Bm Imbert (3) , F. Najioullah (4) , G. Champier (2) , S. Gouarin (5) , J. Carquin (6) , N. Houhou (7) , Isabelle Garrigue (8) , A. Ducancelle (9) , D. Thouvenot (4) , Mc Mazeron (9) , Am Fillet (1)

1 CHU Pitié-Salpêtrière [AP-HP]
2 Teaching hospital
3 Teaching hospital
4 VPV - Virologie et pathogenèse virale
5 Hospital Clemenceau
6 Hôpital Robert Debré
7 Hôpital Bichat - Claude Bernard
8 Hospital Pellegrin
9 Hôpital Lariboisière

We described the natural polymorphism of cytomegalovirus DNA polymerase in 42 unrelated isolates susceptible to ganciclovir, foscarnet, and cidofovir. All variations, including an eight-amino-acid deletion, were located between domains delta-C and II and between domains III and I, suggesting that these specific residues are not involved in enzymatic functions.

Mots clés: Amino Acid Sequence; Amino Acid Substitution; Antiviral Agents/pharmacology; Conserved Sequence; Cytomegalovirus/drug effects/*enzymology/*genetics; DNA-Directed DNA Polymerase/*genetics; Gene Deletion; Humans; Molecular Sequence Data; Phenotype; Polymorphism; Genetic/genetics; Reverse Transcriptase Polymerase Chain Reaction

Domaines: Virologie

L. Auray, S. Alain, K. Gourlain, Bm Imbert, F. Najioullah, et al.. Natural polymorphism of cytomegalovirus DNA polymerase lies in two nonconserved regions located between domains delta-C and II and between domains III and I.. Antimicrob Agents Chemother, 2004, pp.1868. ⟨hal-00123184⟩