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| HAL: hal-00113510, version 1 |
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| FEMS microbiology letters, 261 (2006) 25-31 |
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| Isolationand characterization of functional insertion sequences of rhizobia |
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| Ismael Hernandez-Lucas 1Jose Augusto Ramirez-Trujillo 1 |
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| (2006) |
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| Rhizobia are a group of bacteria that form nodules on the roots of legume host plants. The sequenced genomes of the rhizobia are characterized by the presence of many putative insertion sequences (IS) elements. However, it is unknown whether these IS elements are functional and it is therefore relevant to assess their transposition activity. In this work, several functional insertion sequences belonging to the IS1256, IS3, IS5, IS166, and IS21 families were captured from Rhizobium tropici, Rhizobium sp. NGR234 and Sinorhizobium meliloti, using pGBG1 as a trapping system. In silico analysis shows that homologs of rhizobia mobile elements are present in distantly related genomes, suggesting that Rhizobium IS elements are prone to genetic transfer. |
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| 1: | Departamento de Microbiologia Molecular Instituto de Biotecnologia |
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UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO |
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| 2: | Centro de Ciencias Genomicas |
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UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO |
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| 3: | Ecologie microbienne (EM) |
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CNRS : UMR5557 – INRA : UR1193 – Université Claude Bernard - Lyon I – Ecole Nationale Vétérinaire de Lyon |
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| Subject | : | Life Sciences/Biochemistry, Molecular Biology/Genomics |
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| rhizobia – insertion sequences – transposition. |
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| hal-00113510, version 1 | |
| http://hal.archives-ouvertes.fr/hal-00113510 | |
| oai:hal.archives-ouvertes.fr:hal-00113510 | |
| From: Christine Delorme | |
| Submitted on: Monday, 13 November 2006 16:36:32 | |
| Updated on: Friday, 26 January 2007 10:27:59 | |
