Fourier-transform coherent anti-Stokes Raman scattering microscopy.

Jennifer P. Ogilvie (1) , Emmanuel Beaurepaire (1) , Antigoni Alexandrou (1) , Manuel Joffre (1)

1 LOB - Laboratoire d'optique et biosciences

We report a novel Fourier-transform-based implementation of coherent anti-Stokes Raman scattering (CARS) microscopy. The method employs a single femtosecond laser source and a Michelson interferometer to create two pulse replicas that are fed into a scanning multiphoton microscope. By varying the time delay between the pulses, we time-resolve the CARS signal, permitting easy removal of the nonresonant background while providing high resolution, spectrally resolved images of CARS modes over the laser bandwidth (approximately 1500 cm(-1)). We demonstrate the method by imaging polystyrene beads in solvent.

Domaines: Optique [physics.optics]; Imagerie

Jennifer P. Ogilvie, Emmanuel Beaurepaire, Antigoni Alexandrou, Manuel Joffre. Fourier-transform coherent anti-Stokes Raman scattering microscopy.. Optics Letters, 2006, 31 (4), pp.480-2. ⟨10.1364/OL.31.000480⟩. ⟨hal-00094226⟩