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Article Dans Une Revue Neuroscience Année : 2006

Frequency-dependent modulation of glycine receptor activation recorded from the zebrafish larvae hindbrain

Résumé

In vertebrates, most glycinergic inhibitory neurons discharge phasically at a relatively low frequency. Such a pattern of glycine liberation from presynaptic terminals may affect the kinetics of post-synaptic glycine receptors. To examine this influence, we have analyzed the behavior of glycine receptors in response to repetitive stimulation at frequencies at which consecutive outside-out currents did not superimpose (0.5-4 Hz). Neurotransmitter release was mimicked on outside-out patches from zebrafish hindbrain Mauthner cells using fast flow application techniques. The amplitude of outside-out currents evoked by short (1 ms) repetitive applications of a saturating concentration (3 mM) of glycine remained unchanged for application frequencies1 Hz. Glycine-evoked current simulations using a simple Markov model describing zebrafish glycine receptor kinetic behavior, indicates that this down-regulation of glycine receptor efficacy is due to a progressive accumulation of the receptors in a long lasting desensitization state. Our simulations suggest that this down-regulation can occur even when spontaneous inhibitory currents were generated randomly at a frequency>1 Hz.
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Dates et versions

hal-00086009 , version 1 (19-07-2006)

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J.M. Rigo, P. Legendre. Frequency-dependent modulation of glycine receptor activation recorded from the zebrafish larvae hindbrain. Neuroscience, 2006, 140, pp.389-402. ⟨10.1016/j.neuroscience.2006.01.057⟩. ⟨hal-00086009⟩
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