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Applied and Environmental Microbiology 71 (2005) 6910-6917

An efficient procedure for purification of the obligate intracellular Wolbachia pipientis and representative amplification of its genome by multiple displacement amplification

Patrick Mavingui 1, Van Tran Van 1, Estelle Labeyrie 1, Edwige Rancès 1, Fabrice Vavre 2, Pascal Simonet 1

(2005)

procedure to representatively amplify Wolbachia genome by multiple displacement amplification from limited infected host tissue (0.2 g or 2 x 107 cell line). We obtained sufficient amounts (8 to 10 µg) of DNA of suitable quality for genomic studies with which we demonstrated that the amplified DNA contained all of the Wolbachia loci targeted. In addition, our data indicated that the genome of wRi strain, an obligatory endosymbiont of Drosophila simulans, shared similar overall architecture with its relative strain wMel.

1:	Ecologie microbienne (EM)
	CNRS : UMR5557 – INRA : UR1193 – Université Claude Bernard - Lyon I – Ecole Nationale Vétérinaire de Lyon
2:	HELIX (INRIA Rhône-Alpes)
	INRIA – CNRS : UMR5558 – Université Claude Bernard - Lyon I

Research team: 4

Subject

:

Life Sciences/Biochemistry, Molecular Biology/Genomics

Keyword(s): Wolbachia pipientis – wRi – genome amplification – multiple-displacement amplification

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From: Christine Delorme <>

Submitted on: Monday, 13 November 2006 16:46:53

Updated on: Friday, 26 January 2007 10:24:48